Journal: Frontiers in Oncology

Article Title: Examination of the functions and mechanism of KCP in mediating paclitaxel resistance in cervical squamous carcinoma cells

doi: 10.3389/fonc.2025.1550032

Figure Lengend Snippet: Assessment of optimal KCP knockout targets using paclitaxel resistance in SiHa cell lines.

Article Snippet: The following materials were used: DMEM medium (HyClone, 130701), fetal bovine serum (Hangzhou Sijiqing Company, VS500T), trypsin (GIBCO, 25200-072), paclitaxel (PTX) (PTX, Beijing Xiehe Pharmaceuticals, 141101), KCP -overexpressing lentivirus, KCP gene sgRNA lentivirus (Shanghai Genechem Co.,Ltd), MTT (Shanghai Dingguo Biotechnology Co., Ltd., DH343-2), apoptosis kit (eBioscience, 88-8007), human gene expression profiling chip GeneChip ® PrimeView ® human (Affymetrix, 902487), GeneChip ® Hybridization Wash and Stain Kit (Shanghai GeneChem Co., Ltd), Agilent RNA 6000 Nano Kit (Agilent), TRIzolTM (Shanghai Pu Fei Company, 3101-100), M-mlV (Promega, M1705), dNTPs (Promega, U1240), RNase Inhibitor (Promega, N2115), oligo dT (Shanghai Biotech Company, B0205), Bulge-LoopTM miRNA qPCR Primer Set (Guangzhou Ruibo Company), KCP primary antibodies (Abcam, AB139956), β-actin (Abclonal, RP02968LQ), anti-rabbit secondary antibody (ZSGB-Bio, ZB2301) and SYBR ® Green Master Mixture (TAKARA, DRR041B).

Techniques: Knock-Out

Journal: Frontiers in Oncology

Article Title: Examination of the functions and mechanism of KCP in mediating paclitaxel resistance in cervical squamous carcinoma cells

doi: 10.3389/fonc.2025.1550032

Figure Lengend Snippet: Effect of KCP knockout on SiHa cell colony formation, the experiment was repeated three times (A) : The effect of KCP knockout on cervical squamous cell carcinoma SiHa colony formation. (B) : The inhibition of SiHa cell colony formation after KCP knockdown increased the sensitivity to paclitaxel. sgCtrl: sgRNA-negative control virus-infected SiHa cells; sgCtrl+paclitaxel: paclitaxel-treated cells infected with sgRNA-negative control virus sg KCP : cells infected with sgRNA virus specific to the KCP gene; sg KCP +paclitaxel: paclitaxel-treated cells infected with the sgRNA virus specific to the KCP gene.

Article Snippet: The following materials were used: DMEM medium (HyClone, 130701), fetal bovine serum (Hangzhou Sijiqing Company, VS500T), trypsin (GIBCO, 25200-072), paclitaxel (PTX) (PTX, Beijing Xiehe Pharmaceuticals, 141101), KCP -overexpressing lentivirus, KCP gene sgRNA lentivirus (Shanghai Genechem Co.,Ltd), MTT (Shanghai Dingguo Biotechnology Co., Ltd., DH343-2), apoptosis kit (eBioscience, 88-8007), human gene expression profiling chip GeneChip ® PrimeView ® human (Affymetrix, 902487), GeneChip ® Hybridization Wash and Stain Kit (Shanghai GeneChem Co., Ltd), Agilent RNA 6000 Nano Kit (Agilent), TRIzolTM (Shanghai Pu Fei Company, 3101-100), M-mlV (Promega, M1705), dNTPs (Promega, U1240), RNase Inhibitor (Promega, N2115), oligo dT (Shanghai Biotech Company, B0205), Bulge-LoopTM miRNA qPCR Primer Set (Guangzhou Ruibo Company), KCP primary antibodies (Abcam, AB139956), β-actin (Abclonal, RP02968LQ), anti-rabbit secondary antibody (ZSGB-Bio, ZB2301) and SYBR ® Green Master Mixture (TAKARA, DRR041B).

Techniques: Knock-Out, Inhibition, Knockdown, Negative Control, Virus, Infection

Journal: Frontiers in Oncology

Article Title: Examination of the functions and mechanism of KCP in mediating paclitaxel resistance in cervical squamous carcinoma cells

doi: 10.3389/fonc.2025.1550032

Figure Lengend Snippet: MTT assay of SiHa cell proliferation after infection with sg KCP , the experiment was repeated three times (A) : Comparison of changes in the light absorption measured at a wavelength of 490 nm using a microplate reader after treatment of sgRNA lentivirus-infected SiHa cells with MTT for four hours following incubation with paclitaxel. OD490/fold was the OD490 increase relative to day 1 for each experimental group from day 1 to day 6, representing the proliferation increase for each day. (B) : Comparison of the inhibition of each cell group after the addition of paclitaxel, inhibition rate: [1-OD490/fold(sg KCP /sg KCP + paclitaxel)/OD490/fold(sgCtrl/sgCtrl + paclitaxel)] × 100. (C) :The inhibition rate was significantly higher for the sg KCP group compared to that for the sgCtrl group after the addition of paclitaxel.

Article Snippet: The following materials were used: DMEM medium (HyClone, 130701), fetal bovine serum (Hangzhou Sijiqing Company, VS500T), trypsin (GIBCO, 25200-072), paclitaxel (PTX) (PTX, Beijing Xiehe Pharmaceuticals, 141101), KCP -overexpressing lentivirus, KCP gene sgRNA lentivirus (Shanghai Genechem Co.,Ltd), MTT (Shanghai Dingguo Biotechnology Co., Ltd., DH343-2), apoptosis kit (eBioscience, 88-8007), human gene expression profiling chip GeneChip ® PrimeView ® human (Affymetrix, 902487), GeneChip ® Hybridization Wash and Stain Kit (Shanghai GeneChem Co., Ltd), Agilent RNA 6000 Nano Kit (Agilent), TRIzolTM (Shanghai Pu Fei Company, 3101-100), M-mlV (Promega, M1705), dNTPs (Promega, U1240), RNase Inhibitor (Promega, N2115), oligo dT (Shanghai Biotech Company, B0205), Bulge-LoopTM miRNA qPCR Primer Set (Guangzhou Ruibo Company), KCP primary antibodies (Abcam, AB139956), β-actin (Abclonal, RP02968LQ), anti-rabbit secondary antibody (ZSGB-Bio, ZB2301) and SYBR ® Green Master Mixture (TAKARA, DRR041B).

Techniques: MTT Assay, Infection, Comparison, Incubation, Inhibition

Journal: Frontiers in Oncology

Article Title: Examination of the functions and mechanism of KCP in mediating paclitaxel resistance in cervical squamous carcinoma cells

doi: 10.3389/fonc.2025.1550032

Figure Lengend Snippet: Effect of KCP knockout on SiHa cell apoptosis after treatment with paclitaxel, the experiment was repeated three times (A) : Apoptosis and necrosis rates, UR: upper right (Late apoptosis), LR: lower right (Early apoptosis) (B) : The proportion of early vs. late apoptosis in different group, the differences of early and late apoptosis between sgCtrl vs. sgKCP and sgCtrl+PTX vs. sgKCP+PTX were statistically significant. (C) :The total apoptosis rate ratios were as follows: sgCtrl+paclitaxel/sgCtrl=3.6 and sg KCP +paclitaxel/sg KCP =3.57 (No significant, ns), the late apoptosis rate ratio of KCP knockout SiHa cells was not affected by paclitaxel treatment compared to the control group (ns), but the early apoptosis rate ratio of KCP knockout SiHa cells was lower compared to the control group ( p <0.0001).

Article Snippet: The following materials were used: DMEM medium (HyClone, 130701), fetal bovine serum (Hangzhou Sijiqing Company, VS500T), trypsin (GIBCO, 25200-072), paclitaxel (PTX) (PTX, Beijing Xiehe Pharmaceuticals, 141101), KCP -overexpressing lentivirus, KCP gene sgRNA lentivirus (Shanghai Genechem Co.,Ltd), MTT (Shanghai Dingguo Biotechnology Co., Ltd., DH343-2), apoptosis kit (eBioscience, 88-8007), human gene expression profiling chip GeneChip ® PrimeView ® human (Affymetrix, 902487), GeneChip ® Hybridization Wash and Stain Kit (Shanghai GeneChem Co., Ltd), Agilent RNA 6000 Nano Kit (Agilent), TRIzolTM (Shanghai Pu Fei Company, 3101-100), M-mlV (Promega, M1705), dNTPs (Promega, U1240), RNase Inhibitor (Promega, N2115), oligo dT (Shanghai Biotech Company, B0205), Bulge-LoopTM miRNA qPCR Primer Set (Guangzhou Ruibo Company), KCP primary antibodies (Abcam, AB139956), β-actin (Abclonal, RP02968LQ), anti-rabbit secondary antibody (ZSGB-Bio, ZB2301) and SYBR ® Green Master Mixture (TAKARA, DRR041B).

Techniques: Knock-Out, Control